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KMID : 1007519950040020070
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Food Science and Biotechnology
1995 Volume.4 No. 2 p.70 ~ p.74
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Effects of Environmental Variables on Expression and Secretion of Cloned Invertase from Recombinant Saccharomyces cerevisiae
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Marten, Mark R.
Choi, Chi-Min/Seo, Jin-Ho
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Abstract
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The effects of temperature, medium pH and dissolved oxygen concentration on kxpression and secretion of cloned invertase were investigated for a recombinant yeast system. This system consisted of the baker´s yeast Sacchurmyces cerevisiae (SEY2102) transformed with the 2μ-based plasmid pBR58 which contains the entire SUC2 gene including the promoter, signal sequence and structural gene.
The recombinant yeast grown at 35℃ produced more invertase of 2.2 U/mL/OD than that grown at 25℃ or 30℃. Fermentations done at a controlled pH 6.5 showed a lower growth rate but a higher level of invertase production than those at pH 4.5. Dissolved oxygen (DO) concentrations as low as 25% of saturation were sufficient to maintain invertase production and secretion at levels found at higher DO concentrations.
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